Design, total synthesis, and functional overexpression of the Candida rugosa lipl gene coding for a major industrial lipase
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چکیده
منابع مشابه
Isolation of Candida rugosa lipase isoforms
The yeast Candida rugosa is a convenient source of lipases for science and industry. Crude preparation of Candida rugosa lipase (CRL) consists of several extracellular lipases. Isoenzyme profile depends on the culture or fermentation conditions. All isoforms are coded by the lip pseudogene family; they are monomers of 534 amino acids and molecular weight of about 60 kDa. They share the same cat...
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deployable scissor type structures are composed of the so-called scissor-like elements (sles), which are connected to each other at an intermediate point through a pivotal connection and allow them to be folded into a compact bundle for storage or transport. several sles are connected to each other in order to form units with regular polygonal plan views. the sides and radii of the polygons are...
Kinetic Study of Ethyl Hexanoate Synthesis Using Surface Coated Lipase from Candida Rugosa
Kinetics of lipase-catalyzed esterification of hexanoic acid and ethyl alcohol using the solvent-free system, surface coated lipase from Candida rugosa, had been studied. The effect of various parameters such as reaction time, reaction temperature, reaction kinetics, water removal and feasibility of solvent-free system had been focused. Candida Rugosa lipase was more effective than other li...
متن کاملImmobilization of lipase from Candida rugosa on synthetic polymer beads for use in the synthesis of fatty esters ABSTRACT Lipase from Candida rugosa
Lipase from Candida rugosa was immobilized on three different supports, i.e. Amberlite XAD7, poly(methylmethacrylate) (PMMA) and celite. With the conditions tested, maximum adsorption can be achieved after 30 min. The activities of the immobilized lipases were determined by the esterification reaction of oleic acid and butanol. The immobilized lipases were found to be very effective in the este...
متن کاملCitronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor
The synthesis of citronellyl butyrate by direct esterification reaction catalyzed by immobilized lipase from Candida rugosa was studied in a continuous packed bed reactor using n-hexane as organic solvent. Parameters such as residence time, temperature, and pH were examined. The optimum conversion was obtained at a flow rate of 1 ml/min (residence time 8 min), temperature of 50 °C, and pH 7.5. ...
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ژورنال
عنوان ژورنال: Protein Science
سال: 1998
ISSN: 0961-8368,1469-896X
DOI: 10.1002/pro.5560070618